Laboratory Procedures 2 Theory

Curriculum guideline

Effective Date:
Course
Discontinued
No
Course code
VTEC 1312
Descriptive
Laboratory Procedures 2 Theory
Department
Veterinary Technology
Faculty
Science & Technology
Credits
3.00
Start date
End term
Not Specified
PLAR
No
Semester length
15 Weeks
Max class size
30
Course designation
None
Industry designation
None
Contact hours

Lecture: 3 hours/week

and

Clinical: 2 hours/week

Method(s) of instruction
Lecture
Clinical
Learning activities

Learning activities may include recorded lectures, group work, presentations, case studies, discussion boards, preparation of client handouts, quizzes, written assignments, client communication simulations and guest speakers.  Students will prepare and present case materials based on their small animal hospital clinical experience. Students will practice and be evaluated on laboratory skills during their clinical placement.

Course description
This course further develops veterinary clinical and laboratory skills covered in VTEC 1302 and VTEC 1307 and introduces additional topics. Topics include transfusion medicine, serum chemistry testing, urinalysis, immunology and serology. Cytological and microbiology techniques are reviewed for domestic veterinary species, with an emphasis on pathogens relevant to companion (small) animals. Students must be working in a veterinary clinic or hospital whilst taking this course.
Course content

Transfusion medicine:

  • canine and feline blood-types
  • blood typing procedures
  • cross-matching of blood for transfusion

Urinalysis:

  • sample collection techniques 
  • gross evaluation of urine
  • urine specific gravity
  • biochemical analysis
  • sediment preparation and analysis

Immunological and serological testing:

  • ELISA, serum agglutination and precipitation tests
  • serum protein electrophoresis
  • antibody titres
  • in-clinic test kits for heartworm and feline immunodeficiency and leukemia viruses

Whole blood and serum chemistry:

  • sample collection and handling
  • record keeping and lab requisition forms
  • testing procedures
  • quality control

Endocrine and major organ function testing:

  • thyroid gland function
  • adrenocortical gland function
  • glucose homeostasis
  • hepatic & renal function

Cytology and histopathology:

  • tissue sampling, collection and preservation techniques
  • preparation and staining
  • gross and microscopic tissue and fluid analysis

Microbiology:

  • pathogen growth patterns, morphology, testing techniques and control methods
  • sample collection, including use of culture media
  • gross colony and microscopic organism evaluation including staining techniques
  • biochemical analysis
  • laboratory safety and quality control
  • basics of antimicrobial sensitivity testing
Learning outcomes

Upon successful completion of the course, students will be able to:

  • perform routine laboratory analyser maintenance and quality control techniques;
  • prepare, label, package and store specimens for laboratory analysis;
  • describe veterinary blood types, cross-matching and blood-typing procedures;
  • describe safe handling and preparation of a urine sample, including the steps to a complete urinalysis;
  • discuss the components of a serum chemistry profile and recognize abnormalities;
  • perform a serum chemistry profile using a desk-top chemistry analyser;
  • use a glucometer to obtain blood glucose measurements;
  • describe common serological and immunological veterinary testing procedures;
  • use common ELISA test kits such as feline immunodeficiency and leukemia viruses, heartworm, canine pancreatic lipase and/or giardia SNAP tests;
  • explain proper collection, handling and preparation of blood, tissue and fluid samples for cytological analysis;
  • describe the application of staining techniques, microbiological culture media use and additional testing steps for microscopic organism identification.
Means of assessment

Assessment will be in accordance with the ÌÇÐÄvlog´«Ã½Evaluation Policy.  The instructor will present a written course outline with specific evaluation criteria at the beginning of the semester.

Evaluation will be based on:

Lab evaluations: 10-30% (no single evaluation worth more than 10%)

Assignments: 10-40% (no single assignment worth more than 20%)

Quizzes: 10-20% 

Term test(s): 10-20%

Final examination: 30-40%

Total: 100%

Textbook materials

Consult the ÌÇÐÄvlog´«Ã½Bookstore for the latest required textbooks and materials.  Example textbooks and materials may include:

  • Sirois, M. (Current Edition). Laboratory Procedures for Veterinary Technicians. Elsevier.
Prerequisites

VTEC 1307

Students in the Veterinary Technology program are required to maintain a minimum grade of C in all courses in order to progress in the program.

Corequisites

None

Equivalencies